About

41
Publications
5,615
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
594
Citations
Introduction
Robert Malinowski currently works at the Institute of Plant Genetics, Polish Academy of Sciences. Robert does research in Molecular Biology, Cell Biology and Botany. Their current project is 'Systems biology approach for P. brassicae - Arabidopsis interaction'.
Research Experience
March 2015 - present
Institute of Plant Genetics of the Polish Academy of Sciences
Position
  • ERA Chair BIO-TALENT Head oh the Integrative Plant Biology Group
Description
  • Our main objective is unraveling rules governing the adaptive changes in plant organ growth in response to adverse environmental conditions or the pathogen attack.
January 2013 - February 2015
Polish Academy of Sciences
Position
  • Group Leader Plant Developmental Biology
Description
  • Laboratory of Plant Molecular Biology Main focus of the research is understanding of the impact of cellular changes on the final plant form. This includes also the evolution of developmental programs in plants.
October 2007 - December 2012
The University of Sheffield
Position
Description
  • Leaf Development Plasmodiophora-induced developmental reprogramming

Publications

Publications (41)
Article
Full-text available
Lolium multiflorum/Festuca arundinacea introgression forms have been proved several times to be good models to identify key components of grass metabolism involved in the mechanisms of tolerance to water deficit. Here, for the first time, a relationship between photosynthetic and antioxidant capacities with respect to drought tolerance of these for...
Article
When plants are infected by Plasmodiophora brassicae, their developmental programs are subjected to extensive changes and the resultant clubroot disease is associated with formation of large galls on underground tissue. The pathogen's need to build an efficient feeding site as the disease progresses drives these changes, ensuring successful product...
Article
Full-text available
Plasmodiophora brassicae is a soil‐borne biotroph whose life cycle involves reprogramming of host developmental processes leading to the formation of galls on its underground parts. Formation of such structures involves modification of the host cell cycle leading initially to hyperplasia, increasing the number of cells to be invaded, followed by ov...
Article
Successful biotrophic plant pathogens can divert host nutrition towards infection sites. Here we describe how the protist Plasmodiophora brassicae establishes a long-term feeding relationship with its host by stimulating phloem differentiation and phloem-specific expression of sugar transporters within developing galls. Development of galls in infe...
Article
Full-text available
The expression of two genes encoding xyloglucan endotransglucosylase/hydrolases (XTHs), Cs-XTH1 and Cs-XTH3, was upregulated during the onset of cucumber somatic embryogenesis. As a means of characterising the developmental regulation of these genes, the activity of the respective upstream regulatory regions was investigated in seedlings and somati...
Article
Full-text available
By investigating the metabolism of plants infected by Plasmodiophora brassicae, the agent of clubroot disease, this paper shows the changes in plant metabolites depending on responses of the plant to infection. A nontargeted metabolomic approach was used to obtain metabolic ‘fingerprints’, which were then combined with host transcriptomic data. At...
Article
Full-text available
Background The protist Plasmodiophora brassicae is a soil-borne pathogen of cruciferous species and the causal agent of clubroot disease of Brassicas including agriculturally important crops such as canola/rapeseed (Brassica napus). P. brassicae has remained an enigmatic plant pathogen and is a rare example of an obligate biotroph that resides enti...
Article
Full-text available
The protist Plasmodiophora brassicae is a soil-borne pathogen of cruciferous species and the causal agent of clubroot disease of Brassicas including agriculturally important crops such as canola/rapeseed (Brassica napus). P. brassicae has remained an enigmatic plant pathogen and is a rare example of an obligate biotroph that resides entirely inside...
Article
Full-text available
Clubroot (Plasmodiophora brassicae) is a pathogen of Brassicaceae that causes significant reductions in yield as a consequence of gall formation in the root and hypocotyl of infected plants. The pathogen hijacks host vascular cambium development, and cytokinins are implicated in this process. This paper uses transcriptomics and metabolomics to inve...
Conference Paper
Full-text available
Background / Purpose: It is rare that expansin (a group of extracellular matrix proteins) gene expression, when modulated through a transgenic approach, results in a clear growth response. We investigated the role that expansins play during leaf development and compared that with the growth of hypocotyl and root (data not shown) by using Arabidop...
Article
Full-text available
The leaf is the major organ involved in light perception and conversion of solar energy into organic carbon. In order to adapt to different natural habitats, plants have developed a variety of leaf forms, ranging from simple to compound, with various forms of dissection. Due to the enormous cellular complexity of leaves, understanding the mechanism...
Article
Plasmodiophora brassicae (clubroot) infection leads to reprogramming of host development resulting in the formation of characteristic galls. In this work we explored the cellular events that underly gall formation in Arabidopsis thaliana with the help of molecular markers of cell division (CYCB1:GUS) and meristematic activity (ANT:GUS). Our results...
Article
Full-text available
Understanding the relationship of the size and shape of an organism to the size, shape, and number of its constituent cells is a basic problem in biology; however, numerous studies indicate that the relationship is complex and often nonintuitive. To investigate this problem, we used a system for the inducible expression of genes involved in the G1/...
Article
A classical view is that leaf shape is the result of local promotion of growth linked to cell proliferation. However, an alternative hypothesis is that leaf form is the result of local repression of growth in an otherwise growing system. Here we show that leaf form can indeed be manipulated in a directed fashion by local repression of growth. We sh...
Article
Full-text available
Trifolium alexandrinum L. was transformed with the Arabidopsis HARDY gene that belongs to the stress-related AP2/ERF (APETALA2/ethylene responsive element binding factors) superfamily of transcription factors. The fresh weights of the transgenic lines L2 and L3 were improved by 42 and 55% under drought stress and by 38 and 95% under salt stress com...
Article
Full-text available
Systemin is a wound signaling peptide from tomato that is important for plant defenses against herbivory. The systemin receptor was initially identified as the tomato homolog of the brassinosteroid receptor BRI1, but genetic evidence argued against this finding. However, we found that BRI1 may function as an inappropriate systemin binding protein t...
Article
Full-text available
Expansins are cell wall proteins associated with the process of plant growth. However, investigations in which expansin gene expression has been manipulated throughout the plant have often led to inconclusive results. In this article, we report on a series of experiments in which overexpression of expansin was targeted to specific phases of leaf gr...
Article
Full-text available
The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant cu3 is as sensitive to systemi...
Article
Full-text available
In this study, we introduce the Micro-Electrode Ion Flux Estimation technique as a sensitive and accurate technique to study systemin-induced changes in ion fluxes from isolated nearly intact plant tissues. Our results demonstrate the effectiveness and value of the Micro-Electrode Ion Flux Estimation technique to monitor and characterize those elic...
Article
Full-text available
Parthenocarpy (seedless fruits) is a desirable trait that has been achieved in many plant cultivars. We generated parthenocarpic cucumber fruits by introducing the chimeric DefH9-iaaM construct into the cucumber genome using an Agrobacterium tumefaciens-mediated protocol. The construct consists of the DefH9 promoter from Antirrhinum majus and the i...
Article
Full-text available
Somaclonal variation commonly occurs during in vitro plant regeneration and may introduce unintended changes in numerous plant characters. In order to assess the range of tissue-culture-responsive changes on the biochemical level, the metabolic profiles of diploid and tetraploid cucumber R1 plants regenerated from leaf-derived callus were determine...
Article
Full-text available
Defined changes in the cell wall directed by many proteins accompany every morphogenetic process in plants. Xyloglucan endotransglucosylase/hydrolase proteins (XTH; EC 2.4.1.207) have the potential to modify the hemicellulose matrix within the cell wall. Cs-XTH1 and Cs-XTH3 genes, which encode XTH proteins, were found among numerous genes that are...
Article
Full-text available
The expression pattern of a Solanum sogarandinum pGT::Dhn10 gene fusion encoding a dehydrin DHN10 protein and the potential role of that protein in cold tolerance in cucumber were analysed in three T1transgenic lines. An accumulation of Dhn10 mRNA was detected in the leaves, cotyledons, hypocotyls and roots of the transgenic seedlings both under th...
Article
A phenotypic segregation of kanamycin resistance conferred by nosnptII was investigated in two groups of transgenic cucumber lines one containing the PR-2duidA-nosnptII construct and the other with the thaumatinII-nosnptII construct to the third and fifth generation, respectively. Expression of the nptII gene was more stable and predictable in the...
Article
Full-text available
This review presents recent data about cell wall involvement in plant embryogenesis. During plant development, the cell wall is subjected to precise regulation. During this process a bidirectional information exchange between the cell wall and the protoplast is observed. The cell wall also mediates in the cell-cell (apoplastic) and cell to cell (sy...
Article
Tomato ls mutant characterised by suppressed lateral shoots, abnormal flowers and low level of endogenous cytokinins, was transformed with a Agrobacterium tumefaciens strain ACS101 carrying an ipt gene under a heat shock promoter. Of the 62 rooted shoots that were obtained, most exhibited unchanged ploidy levels. The PCR analysis confirmed that 76%...

Questions and Answers

Question & Answers (6)

Network

Cited By

Projects

Projects (3)
Archived project
Molecular basis of somatic embryogenesis of cucumber
Project
The Department of Integrative Plant Biology at the Institute of Plant Genetics, Polish Academy of Sciences will reinforce the research potential through: 1. joining the European Research Area, 2. employment of excellent scientists in plant biology and 3. strenghthening international co-operation with leading European researchers and laboratories.
Project
Holistic understanding of the P. brassicae driven host reprogramming.