Human adenoviruses (HAdV) are causing a broad spectrum of diseases. One of the most severe forms of adenovirus infection is a disseminated disease resulting in significant morbidity and mortality. Several reports in recent years have identified HAdV-31 from species A (HAdV-A31) as a cause of disseminated disease in children following haematopoetic...

In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recomb...

Human adenoviruses (HAdV) are responsible for a wide spectrum of diseases. The neutralization epsilon determinant (loops 1 and 2) and the hemagglutination gamma determinant are relevant for the taxonomy of HAdV. Precise type identification of HAdV prototypes is crucial for detection of infection chains and epidemiology. epsilon and gamma determinan...

The penton base is a major capsid protein of human adenoviruses (HAdV) which forms the vertices of the capsid and interacts with hexon and fiber protein. Two hypervariable loops of the penton are exposed on the capsid surface. Sequences of these and 300 adjacent amino acid residues of all 51 HAdV and closely related simian adenoviruses were studied...

Traumatic amputation of a thumb with bone loss leaves a patient in severe disability. Reconstructive procedures are restricted by limited shape and have the disadvantage of severe donor-site morbidity. To overcome these limitations, we used a tissue engineering approach to create a distal thumb bone phalanx, combining magnetically sorted 133+ human...

Various systems and methods for creating, storing, structuring, displaying, enhancing, and/or referencing publications and related content in an online user network are described. In some embodiments, a plurality of “anchors” are dispersed throughout the document, allowing document portions to be referenced in terms of the anchor(s) associated with...

This file is an aligned fasta file including the fiber knob (nucleotide) gene from 51 human adenoviruses (1-51) (Species A-F)

This file is an aligned fasta file including the loop1 area of the hexon (nucleotide) gene from 51 human adenoviruses (1-51) (Species A-F).

The fiber shaft of human adenoviruses (HAdVs) is essential for bringing the penton base into proximity to the secondary cellular receptor. Fiber shaft sequences of all 53 HAdV types were studied. Phylogeny of the fiber shaft revealed clustering corresponding to the HAdV species concept. An intraspecies recombination hot spot was found at the shaft/...

Regenerative technology promises to alleviate the problem of limited donor supply for bone or organ transplants. Most expensive and time consuming is cell expansion in laboratories. We propose a method of magnetically enriched osteoprogenitor stem cells, dispersed in self-assembling hydrogels and applied onto new ultra-high resolution, jet-based, t...

To examine trabecular microarchitecture with high-resolution flat-panel volume computed tomography (CT) and bone mineral density (BMD) with dual-energy x-ray absorptiometry (DXA) in adolescent girls with anorexia nervosa (AN) and to compare these results with those in normal-weight control subjects. The study was approved by the institutional revie...

Gating in small-animal CT imaging can compensate artefacts caused by physiological motion during scanning. However, all published gating approaches for small animals rely on additional hardware to derive the gating signals. In contrast, in this study a novel method of intrinsic respiratory gating of rodents was developed and tested for mice (n=5),...

Ziele: Evaluation einer digitalen Subtraktionstechnik für die Elimination von überlagernden Knochen und Wandverkalkungen. Methode: In 15 Patienten wurde erg?nzend zu einer CT-Angiographie (CTA) eine native Niedrigdosis-Untersuchung durchgeführt. Alle CTA-Untersuchungen erfolgten mit folgendem Scanprotokoll: Schichtkollimation/Rekonstruktionsinkreme...

Availability of grafts and morbidity at the donor site limit autologous transplantation in patients requiring bone reconstruction. A tissue-engineering approach can overcome these limitations by producing bone-like tissue of custom shape and size from isolated cells. Several hydrogels facilitate osteogenesis on porous scaffolds; however, the relati...

Current techniques to measure computed tomography (CT) spatial resolution use separate methods for in-plane and out-of-plane directions. The growing use of near-isotropic voxel size necessitates a new single method that inherently measures resolution in any direction. We introduce a method using a set of numerous glass microspheres suspended in a s...

In a 4-week period, 12 patients contracted adenoviral keratoconjunctivitis. Eight of these patients had visited the same ophthalmologist's practice before onset of symptoms. Adenovirus was detected in swab specimens obtained from 9 patients. Sequence-based typing of 2 isolates revealed type 22/H8. This is, to our knowledge, the first report of a ke...

Human adenovirus (HAdV) infections are increasingly frequent and potentially fatal as a disseminated disease in highly immunocompromised patients. Determining the in vitro sensitivity of HAdV to antiviral agents is not an easy task because HAdV CPE reduction assays are difficult to interpret and may take more than 1 week. We developed a phenotypic...

Precise typing of human adenoviruses (HAdV) is fundamental for epidemiology and the detection of infection chains. As only few of the 51 adenovirus types are associated with life- threatening disseminated diseases in immunodeficient patients, detection of one of these types may have prognostic value and lead to immediate therapeutic intervention. A...

Trabecular bone is a material of choice for reconstruction after trauma and tumor resection and for correction of congenital defects. Autologous bone grafts are available in limited shapes and sizes; significant donor site morbidity is another major disadvantage to this approach. To overcome these limitations, we used a tissue engineering approach...

On or before Apr 6, 2016 this sequence version replaced gi:107954418, gi:107954419, gi:107954417.

PURPOSE Research in tissue-engineering traditionally relies on protein transcription and expression (PTE) analysis to demonstrate tissue growth. For demonstrating bone growth, mechanical properties of the construct are also used for the same purpose. Using an ultra-high resolution, flat-panel Volume CT (VCT), we demonstrate the first-ever imaging o...

PURPOSE To propose a standardized and quantitative assessment of image quality and to assess differences between three different image reconstruction protocols for coronary MDCT angiography. METHOD AND MATERIALS We assessed 20 normal coronary segments from two patients who underwent standard coronary MDCT (Siemens Sensation 64, 64x0.6 mm collimati...

PURPOSE Spatial resolution of a CT scanner is typically characterized by its modulation transfer function and slice selectivity profile. These methods are inherently anisotropic and fail to fully describe the capabilities of recently introduced volume CT scanners (VCT). A new method is required to describe the spatial resolution of VCT that is inhe...